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英文名字 Urine Cell-Free Circulating RNA Purification Mini Kit
中文名称 尿液无细胞循环RNA纯化小提试剂盒(提取试剂盒)
供应商 Norgen Biotek
产品货号 NGB-56900
产品报价 ¥4737.9816/50preps
购买方式 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868。
产品新闻
背景资料 Recent evidence indicates that cell-free circulating RNA (cf-RNA) including exosomal RNA in urine contains valuable information for the discovery of biomarkers that can help for the early detection of certain cancer types and for monitoring the disease status as well as for the detection of any infectious pathogens. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acid for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum but with a lower concentration; (3) Nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).
产品描述 This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 250 礚 to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
产品特点 Isolate all sizes of circulating and exosomal RNA, including microRNA; Versatile urine input ranging from 250 礚 to 2 mL; No phenol extractions; No carrier RNA; Bind and elute all RNA irrespective of size or GC content, without bias; Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 50 礚 to 100 礚; Purify high-quality RNA in 25 - 30 minutes.
保存建议 溶液密封室温(15-25°C)保存,未开封至少保存2年。具体保存情况收到产品后请按说明书存放。
产品说明书
分享保存
其他 Norgen Biotek目前的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。拥有超过二十个专利,作为一家成长型的公司,其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。此外,Norgen Biotek最近推出了100多款用于核酸诊断的试剂盒,包括从尿液、血浆和血清中诊断人类病原体的试剂盒,诊断食源性病原体的试剂盒和诊断植物病原体的试剂盒。这些试剂盒仅供研究使用,非常适合用于监测耐药病原体,流行病学研究,病原体现场监测和调查。Norgen Biotek还致力于为科研和临床试验提供高质量的分子诊断测试。

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