英文名字 Urine Cell-Free Circulating DNA Purification Mini Kit
中文名称 尿液无细胞循环DNA纯化小提试剂盒(提取试剂盒)
供应商 Norgen Biotek
产品货号 NGB-56600
产品报价 ¥2760.38928/50preps
购买方式 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868。
产品新闻
背景资料 Recent evidence indicates that cell-free circulating DNA (cf-DNA) contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood). Urine cell-free circulating DNA (cf-DNA) has been utilized for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases, as well as for investigating fetal DNA that is normally present in the maternal blood. This cf-DNA is usually present as short fragments, generally between 150 and 250 bp or its duplicates, and is derived either from the apoptotic process, necrotic process or from the fetus.
产品描述 This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples from volumes ranging from 250 礚 to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.
产品特点 Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples; Isolate viral DNA; Versatile urine input volumes (250 礚 - 2 mL); Concentrate circulating DNA into a flexible elution volume ranging from (50 礚 - 100 礚); Isolate inhibitor-free cell-free circulating DNA; Purify high-quality DNA in 15-20 minutes; Compatible with Norgen’s Urine Preservative and other commercially available urine preservatives.
保存建议 溶液密封室温(15-25°C)保存,未开封至少保存2年。具体保存情况收到产品后请按说明书存放。
产品说明书
分享保存
其他 Norgen Biotek目前的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。拥有超过二十个专利,作为一家成长型的公司,其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。此外,Norgen Biotek最近推出了100多款用于核酸诊断的试剂盒,包括从尿液、血浆和血清中诊断人类病原体的试剂盒,诊断食源性病原体的试剂盒和诊断植物病原体的试剂盒。这些试剂盒仅供研究使用,非常适合用于监测耐药病原体,流行病学研究,病原体现场监测和调查。Norgen Biotek还致力于为科研和临床试验提供高质量的分子诊断测试。

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